investigator_user investigator user funding collaborators pending menu bell message arrow_up arrow_down filter layers globe marker add arrow close download edit facebook info linkedin minus plus save share search sort twitter remove user-plus user-minus
  • Project leads
  • Collaborators

In Vivo Multiphoton Based Imaging of Complex Cancer Cell Behavior

Vladislav Verkhusha

0 Collaborator(s)

Funding source

National Institutes of Health (NIH)
Recent results have led many to propose a microenvironment-dependent model for initiation ofmigratory and disseminating tumor cell behavior at both the primary tumor and within targetorgans that is not stably specified by genetic mutation and that is transient in time and space. This view is called the microenvironment model of metastasis. The testing of this model hasbeen hampered in part by the lack of high-resolution in vivo microscopy methods andgenetically-encoded fluorescent probes for tumor deep-tissue imaging that allow definitiveidentification of the microenvironments involved in initiating the migratory and disseminatingtumor cell phenotype. Equally problematic are the limitations of standard analyses of expressionprofiles. Standard analysis of expression profiles in cancer involves identifying consistently up-and down- regulated genes. While these techniques are likely to identify sets of genes directlywithin affected networks, our previous theoretical results have shown that major perturbations(of which cancer is one) cause expression changes far beyond the pathway involved. Crucially,these more distant changes will be highly variable depending on the genetic background, thustumor expression profiles are expected to be greatly dissimilar between individuals. Using thishypothesis we propose a novel systems-level analysis of cancer (SLAC), which identifies keygenes based upon increase in expression variability, and which in turn offers the possibility ofdiscovering highly non-intuitive pathway interactions connected with microenvironment regulation of breast cancer progression. By combining the multiphoton high-resolutionmicroscopy having the wide range of excitation wavelengths with the proposed multicolor far-redfluorescent probes as versatile as conventional GFP we will advance deep-tissue cell labelingand imaging of tumor cells dynamics in vivo. This approach will make possible the intravitalimaging of simultaneously up to six genetically-encoded colors in tumor studies. This in turn will provide a way to discriminate and subsequently isolate the tumor cells of multiple metastatic phenotypes based on the fluorescent color-encoded expression patterns. By correlating the behavior and fate of migrating and disseminating tumor cells obtained by the multiphotonimaging at a single-cell level with SLAC analysis of expression profiles of these cells, we willidentify the key genes driving tumor cell behaviors involved in metastasis such as cell migrationand dissemination.

Related projects